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Figure/Table detail
Reverse phase high-performance liquid chromatography (RP-HPLC) system for analysis of vitamin D3 soft capsules with soybean oil matrix
LI Meng-han▲, DING Xue-mei▲, LIANG Jian-ying△
jms
, 2019, 46(
01
): 23-31. DOI:
10.3969/j.issn.1672-8467.2019.01.004
6
Effect of ethanol or methanol addition in acetonitrile as mobile phase on recovery of vitamin D3 soft capsules with oil matrix (
n
=6)
Other figure/table from this article
R:-CHH
3
(CH
2
)
3
CH(CH
3
)
2
Photochemical and thermal isomers of vitamin D3
A:Thermal degradation; B:Photo degradation; C:Thermal and photo degradation.Peak 1:Trans-vitamin D3;Peak 2:Pre-vitamin D3;Peak 3:Tachysterol; Peak 4:Vitamin D3.
Chromatograms of forced degradation samples
a:Vitamin D3 (λ
max
=262 nm); b:Pre-vitamin D3 (λ
max
=260 nm); c:Trans-vitamin D3 (λ
max
=276 nm); d:Tachysterol (λ
max
=280 nm).λ
max
:Maximum absorption wavelength.
UV absorption spectrums of vitamin D3 and its three isomers
1
Effect of mobile phase composition on various chromatographic parameters
A and B:a-e,
V
(ACH):
V
(MeOH)=99:1, 98:2, 97:3, 90:10 and 80:20;C:a-c,
V
(ACH):
V
(H
2
O)=95:5, 90:10 and 85:15.MeOH:Methanol; ACN:Acetonitrile; H
2
O:Water.
Chromatograms of sample 4 with various proportions of methanol (A), ethanol (B) and water (C) in acetonitrile as mobile phase
a:100%ACN; b-e:
V
(ACN):
V
(MeOH)=99:1, 98:2, 97:3, 90:10, respectively; f-i:
V
(ACN):
V
(EtOH)=99:1, 98:2, 97:3, 90:10, respectively.
Asymmetry of vitamin D3 peak using different mobile phases
A:100% acetonitrile; B:
V
(acetonitrile):
V
(H
2
O)=95:5.a:Degradation sample (sample 3);b:Blank matrix of vitamin D3 soft capsule.
Chromatograms of degradation sample and blank matrix with 100% acetonitrile and
V
(acetonitrile):
V
(H
2
O)=95:5 as mobile phase
