Fudan University Journal of Medical Sciences

Reverse phase high-performance liquid chromatography (RP-HPLC) system for analysis of vitamin D3 soft capsules with soybean oil matrix

LI Meng-han▲, DING Xue-mei▲, LIANG Jian-ying△

jms, 2019, 46(01): 23-31. DOI: 10.3969/j.issn.1672-8467.2019.01.004

Mobile phase composition	Peak	RT (min)	Resolution	α	Asymmetry	k'
RT:Retention time; α:Separation factor; k':Capacity factor.
ACN	1	26.04			1.02	9.54
	2	30.20	5.30	1.16	1.02	11.23
	3	31.54	1.55	1.04	1.00	11.77
	4	35.16	3.73	1.11	1.36	13.23
V (ACN):V (EtOH)=99:1	1	25.64			0.88	9.51
	2	29.12	3.27	1.14		10.93
	3	29.97	0.78	1.03		11.28
	4	32.52	2.14	1.08	0.88	12.33
V (ACN):V (EtOH)=98:2	1	25.15			0.96	9.27
	2	28.29	3.62	1.12		10.55
	3	28.83	0.62	1.02		10.77
	4	30.85	2.14	1.07	0.96	11.59
V (ACN):V (EtOH)=97:3	1	24.72			0.99	9.05
	2+3	27.95	2.92	1.13	0.80	10.36
	4	29.71	1.49	1.06	0.96	11.08
V (ACN):V (EtOH)=90:10	1	19.94			1.20	6.98
	2+3	21.62	2.67	1.08		7.65
	4	22.34	1.10	1.03	1.10	7.94
V (ACN):V (EtOH)=80:20	1	15.35			1.10	5.09
	2+3+4	16.63	2.43	1.08	0.84	5.60
V (ACN):V (MeOH)=99:1	1	25.46			0.94	9.43
	2	28.92	3.36	1.14		10.85
	3	29.78	0.81	1.03		11.20
	4	32.24	2.24	1.08	0.93	12.21
V (ACN):V (MeOH)=98:2	1	24.94			0.90	9.18
	2+3	28.48	2.67	1.14	0.75	10.62
	4	30.41	1.34	1.07	0.90	11.41
V (ACN):V (MeOH)=97:3	1	24.61			0.93	9.00
	2+3	27.69	2.64	1.13	0.87	10.26
	4	29.47	1.43	1.06	0.91	10.98
V (ACN):V (MeOH)=90:10	1	22.56			1.21	8.32
	2+3	24.62	2.72	1.09		9.17
	4	25.59	1.21	1.04	1.02	9.57
V (ACN):V (MeOH)=80:20	1	21.16			1.10	7.78
	2+3+4	23.22	3.08	1.10	0.77	8.63
V (ACN):V (H₂O)=95:5	1	43.52			1.07	18.34
	2+3	48.29	3.91	1.11	1.10	20.46
	4	53.08	3.25	1.10	1.11	22.59
V (ACN):V (H₂O)=90:10	1	58.71			1.10	17.40
	2+3	63.23	3.45	1.08	0.92	18.82
	4	67.54	3.05	1.07	1.08	20.17
V (ACN):V (H₂O)=85:15	1	87.56				25.53
	3	91.20	2.70	1.04		26.64
	2	93.09	1.27	1.02	1.11	27.21
	4	98.09	2.81	1.05	1.08	28.72

1 Effect of mobile phase composition on various chromatographic parameters

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