XU Yan;ZOU Jianzhou;LIU Zhonghua;JIANG Suhua;XU Shaowei;SHEN Bo;DING Xiaoqiang△
Objective To measure the nuclear factor kappa B (NF-κB) activity of peripheral blood mononuclear cell (PBMC) and to observe the relationship between NF-κB activity and cardiovascular disease (CVD) in maintenance hemodialysis (MHD) patients.Methods Blood was obtained from 32 MHD patients before and after undergoing hemodialysis and 12 agematched healthy subjects.Nuclear extracts NF-κB activity of PBMC and plasma pentraxin3 (PTX3) were measured by enzymelinked immunosorbant assay (ELISA).Pearson correlation and linear regression were used to assess the relationship between NF-κB activity and other laboratory parameters.Binary logistic regression was used to assess the association of NF-κB activity and CVD in MHD patients.Receiver operating characteristic (ROC) curves were used to assess the association with NF-κB activity,PTX3,high sensitive Creactive protein (hsCRP) and CVD in MHD patients.Results NF-κB activity of PBMC,PTX3 and hsCRP were significantly higher in MHD patients compared with controls.After a single HD session NF-κB activity of PBMC and PTX3 increased acutely.Patients with CVD had higher NF-κB activity than nonCVD patients.In all MHD patients NF-κB activity correlated positively with white blood cell count (r=0.454,P=0.009),hsCRP (r=0.590,P<0.001),PTX3(r=0.680,P<0.001),cardiac troponin T (r=0.409,P=0.020) and carotid artery intimamedia thickness (r=0.541,P=0.011).Linear regression analysis showed that white blood cell count (β=0.338,P=0.028),hsCRP (β=0.440,P=0.005),PTX3 (β=0.487,P=0.004) and carotid artery intimamedia thickness (β=0.453,P=0.032) were independently associated with NF-κB activity.High NFκB activity was positively and independently associated with CVD (OR=8.47,P=0.022).ROC curves analysis showed the association between NF-κB activity and CVD was more closely than PTX3 and hsCRP.The areas under the curve for NFκB activity,PTX3 and hsCRP were 0.791 (95%CI 0.633-0.950,P=0.006),0.785 (95%CI 0.628-0.943,P=0.007) and 0.711 (95%CI 0.514-0.907,P=0.046), respectively.Conclusions NF-κB activity of PBMC is markedly elevated in MHD patients; hemodiaysis (HD) procedure induces NF-κB activation; NF-κB activity of PBMC is associated with CVD and could be a useful marker of inflammation and risk factor of CVD in MHD patients.
