Objective To investigate the effects of 14-3-3 τ protein secreted by human trophoblast cells on the expression of integrin α_vβ₃ protein in human endometrial stromal cells. Methods Small interference RNA (siRNA) targeting 14-3-3 τ mRNA was transfected into human trophoblast cell line (BeWo cells). The expression of 14-3-3 τ protein in the BeWo cells and its culture-medium were detected by Western blot respectively. Then the BeWo cells transfected with 14-3-3 τ siRNA were co-cultured with human endometrial stromal cells (ESC). The expression of 14-3-3 τ protein in the co-culture-medium and integrin αvβ3 protein in ESC were detected by Western blot respectively. Results Compared with negative control of siRNA, the expression of 14-3-3 τ protein in both BeWo cells and culturemedium were significantly inhibited by 14-3-3 τ siRNA. In the co-culture system, BeWo cells downregulated by 14-3-3 τ siRNA resulted in an increased expression of integrin α_vβ₃ protein in ESC (P<0.05). The expression of integrin α_vβ₃ protein was significantly inhibited in the ESC co-cultured with human 14-3-3 τ recombinant protein (P<0.05). Conclusions 14-3-3 τ protein can be secreted by human trophoblast cells, and may regulate the receptivity of human endometrial stromal cells.