Objective To study cyclin T1 and β-catenin’s expression and their roles in renal clear cell carcinoma (RCCC). Methods Cyclin T1 and β-catenin were investigated in 40 cases of RCCC and in the normal paratumor tissue by immumohistochemical staining. Non-condition Logistic analysis and χ² test were used to search the correlating factors of cyclin T1 expression. Results In all the 40 cases of RCCC，37 (92. 5%) showed positive cyclin T1 staining, among which 14 cases (35%) showed strong positive staining. The rate of positive expression of cyclin T1 staining in RCCC was significantly higher than that in normal kidney tissue (χ²=9·038, P=0·003). Thirty-six cases (90%) showed positive (β-catenin expression in cytoplasm, among which 18 cases (45%) showed strong positive expression. χ² test showed that cyclin T1 expression correlated with tumor grade or cytoplasmic β-catenin expression (P=0.039, 0.014 respectively), and had negative relations with gender, age, tumor size or tumor stage (P>0.05). Non-condition Logistic analysis showed that cyclin T1 only correlated with cytoplasmic β-catenin (P=0.018). Conclusions cyclin T1, which is up-regulated in renal clear cell carnoma，correlates with the tumor differentiation and cytoplasmic β-catenin level, and can he one of the targets of β-catenin signal.