目的 探讨聚肌胞联合E7(44-62)刺激对自然杀伤细胞（natural killer cell，NK细胞）的影响。方法 NK92细胞分别给予培养液（C组）或聚肌胞（聚肌胞组）或聚肌胞联合E7(44-62)（联合组）刺激；检测刺激前后的NK92细胞的Toll样受体3、TNF-α和INF-γ mRNA水平以及肿瘤细胞杀伤活性。结果 刺激1 h后，联合组的Toll样受体3表达明显高于C组和聚肌胞组；刺激1 h后，联合组的TNF-α mRNA水平显著高于C组和聚肌胞组（P均<0.01）；联合组的INF-γ mRNA水平显著高于C组和聚肌胞组（P均<0.05）；刺激24 h后，联合组NK92细胞的肿瘤细胞杀伤活性显著高于C组和聚肌胞组（P均<0.01）。结论 聚肌胞联合E7(44-62)通过提高NK92细胞的Toll样受体3表达，同时增加TNF-α和INF-γ基因的水平，从而增强抗肿瘤反应。

Objective To investigate the effect of poly(I:C)-adjuvanted E7(44-62) on natural killer cells-mediated anti-tumor immunity. Methods NK92 cells were treated with medium (C group), poly(I:C) [poly(I:C) group] or poly(I:C)-adjuvanted E7(44-62) (combined group). Then, toll-like receptor 3 expression, TNF-α and INF-γ mRNA levels and tumor cell-killing activity were measured before and after treatment. Results One hour after treatment, the toll-like receptor 3 expression in the combined group was obviously higher than those of C group and poly(I:C) group. One hour after treatment, the level of TNF-α mRNA in the combined group was significantly higher than those of C group and poly(I:C) group (all P<0.01); the level of INF-γ mRNA in the combined group was significantly higher than those of C group and poly(I:C) group (all P<0.05). One day after treatment, the tumor cell-killing activity in the combined group was significantly higher than those of C group and poly(I:C) group (all P<0.01). Conclusions Poly(I:C)-adjuvanted E7(44-62) can enhance ntural killer cells-mediated anti-tumor immunity by increasing the expression of Toll-like receptor 3, TNF-α and INF-γ gene.