丙泊酚对颈髓损伤大鼠心肌的保护作用

徐振东;石学银;何星颖;邹最;李盈科

复旦学报(医学版) ›› 2008, Vol. 35 ›› Issue (5) : 711-0.

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复旦学报(医学版) ›› 2008, Vol. 35 ›› Issue (5) : 711-0.
论著

丙泊酚对颈髓损伤大鼠心肌的保护作用

  • 徐振东1, 石学银2, 何星颖2, 邹最2, 李盈科2
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The protective effect of propofol on myocardium after cervical spinal cord injury in rats

  • XU Zhen-dong1Δ,SHI Xue-yin2,HE Xing-ying2,Zou Zui2,Li Ying-ke2.
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摘要

目的 研究丙泊酚对高位脊髓损伤(spinal cord injury, SCI)大鼠心肌的保护作用。方法 SD大鼠(96只)随机分为3组:对照组(未损伤组,输入乳酸钠林格氏液12mL&#8226;kg-1&#8226;h-1),乳酸钠林格氏液组(C7脊髓损伤后输入乳酸钠林格氏液12mL&#8226;kg-1&#8226;h-1),丙泊酚组(C7脊髓损伤后输入丙泊酚15mg&#8226;kg-1&#8226;h-1)。在损伤后1h测定心肌c-fos蛋白的表达;4h、24h测定心肌酶;72h观察心肌镜下结构的变化。每组每个时点为8只大鼠(n=8)结果 同对照组相比,输注乳酸钠林格氏液的损伤组大鼠心肌c-fos蛋白表达增加,心肌酶增高(P<0.05),超微结构有异常改变。丙泊酚组上述改变亦较对照组明显,但显著低于乳酸钠林格氏液组(P<0.05)。结论 高位SCI后的大鼠存在心肌损伤,早期使用丙泊酚可以显著减轻心肌的损伤。

Abstract

Objective To study the protective effect of propofol on myocardium after cervical spinal cord injury(SCI)in rats. Methods A cervical laminectomy extending from C6-T1 was performed and the experimental acute cervical 7 spinal cord injury model was established by modified Allen’s method.Totally 96 male Sprague Dawley rats were randomly divided into three groups: control group(sham operation); lactate Ringer’s injection group(infusion with lactate Ringer’s injection at a rate of 12 mL&#8226;kg-1&#8226;h-1 over 1 hour after SCI); propofol group(infusion with propofol at a rate of 15 mg&#8226;kg-1&#8226;h-1 over 1 hour after SCI). At 1h after SCI, c-fos protein in myocardium was detected by immuno-histochemical methods. At 4h and 24h after SCI, we measured serum LDH,CK and CK-Mb level. At 72h, myocardial specimen was sampled from left ventricle to observe structure under light and electron microscope. Results c-fos protein expression and cardiac enzymes level in lactate Ringer’s injection group were higher than those in the control group and propofol group(P<0.05. Ultrastructure of myocardial tissue presented abnormal changes in SCI groups, but the changes in propofol group were milder. Conclusion High level spinal cord injury could induce

关键词

脊髓损伤 / 丙泊酚 / 心肌酶谱 / c-fos

Key words

spinal cord injuries / propofol / myocardial enzymes / c-fos

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徐振东;石学银;何星颖;邹最;李盈科. 丙泊酚对颈髓损伤大鼠心肌的保护作用[J]. 复旦学报(医学版), 2008, 35(5): 711-0
XU Zhen-dongΔ;SHI Xue-yin;HE Xing-ying;Zou Zui;Li Ying-ke. . The protective effect of propofol on myocardium after cervical spinal cord injury in rats [J]. Fudan University Journal of Medical Sciences, 2008, 35(5): 711-0
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