Expression and significance of eNOS and HO-1 in heart and lung tissues of hyperlipidemia mice
Jiang Yi-feng1, Cao Xiao-e2△, Wang Li1, Zhao Feng-di1, Yin Lian-hua1
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1Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai 200032, China; 2Department of Physiology, Shanghai Medical Workers College, Shanghai 200237, China
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1900-01-01
1900-01-01
2008-05-20
发布日期
2013-11-29
摘要
目的 观察高脂饲料喂养的小鼠心脏和肺组织中内皮型一氧化氮合酶（endothelial nitric-oxide synthase, eNOS）及血红素氧合酶-1（heme oxygenase-1, HO-1）的含量变化，探讨高脂血症对eNOS和HO-1表达的影响。方法 高脂饮食组（n=8）和普通饮食组（n=8）C57BL/6小鼠分别经高脂饲料和普通饲料喂养18周后，测血清总胆固醇（total cholesterol, TC）、甘油三酯（triglyceride, TG）、高密度脂蛋白（high density lipoprotein, HDL）和低密度脂蛋白（low density lipoprotein, LDL）含量，采用Real-Time PCR检测心脏和肺组织中eNOS及HO-1的mRNA水平，以免疫组化方法检测其蛋白表达。结果 高脂饮食组TC、TG和LDL明显高于普通饮食组，HDL则低于普通饮食组；高脂饮食组心脏和肺组织中eNOS的mRNA和蛋白水平低于普通饮食组，HO-1的mRNA和蛋白水平高于普通饮食组。结论 高脂饲料喂养能使小鼠形成高脂血症，并引起心脏和肺组织中eNOS的表达降低，同时HO-1的表达升高。
Abstract
Objective To explore the effects of hyperlipidemia in high-fat diet mice on expression of endothelial nitric-oxide synthase (eNOS) and heme oxygenase-1 (HO-1) in heart and lung tissues. Methods C57BL/6 mice were fed a high-fat diet as high-fat diet group (n=8, high-fat diet group) and were fed a normal diet as control (n=8, normal diet group). 18 weeks later, serum lipid profiles including total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL) and low density lipoprotein (LDL) were detected. eNOS and HO-1 mRNA expression in heart and lung tissues were determined by Real-Time PCR, as well as protein expression by immunohistochemistry. Results Levels of serum TC, TG and LDL significantly increased in high-fat diet group, while HDL decreased. mRNA and protein expression of eNOS in heart and lung tissues reduced in high-fat diet group, while HO-1 increased. Conclusion High-fat diet may lead to hyperlipidemia in mice, which may down-regulate eNOS expression and up-regulate HO-1 expression in heart and lung tissues.
Jiang Yi-feng;Cao Xiao-e△;Wang Li;Zhao Feng-di;Yin Lian-hua.
Expression and significance of eNOS and HO-1 in heart and lung tissues of hyperlipidemia mice[J]. Fudan University Journal of Medical Sciences, 2008, 35(3): 342-0
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