目的 探讨剪切波超声弹性成像（shear wave elastography，SWE）定量评价新西兰白兔阴茎海绵体纤维化程度的可行性。方法 随机选取不同周龄阴茎海绵体正常和阴茎海绵体发生纤维化的新西兰白兔各10只进行阴茎SWE检查，测量阴茎海绵体的剪切波弹性量化测值（shear wave elastic quantitative measurement，SWQ）。SWE检查完成后，切取阴茎组织进行苏木精-伊红染色、苦味酸-天狼星红染色和免疫组化染色，测定以下反映阴茎海绵体纤维化程度的指标：海绵体内胶原纤维/平滑肌细胞（the ratio of collagen fiber to smooth muscle cell，CF/SMC）比值、纤维连接蛋白（fibronectin，FN）和Ⅲ型胶原纤维（collagen type Ⅲ，Col Ⅲ）含量。比较两组间周龄、体质量、SWQ差异，并采用Spearman秩相关检验对所有兔阴茎海绵体的SWQ与CF/SMC、FN和Col Ⅲ进行相关性分析。结果 两组间周龄和体质量差异无统计学意义；SWQ与CF/SMC、FN和Col Ⅲ均呈高度正相关（P值均<0.001），相关系数r分别为0.760、0.888和0.801。结论 SWE可以对新西兰白兔阴茎海绵体的纤维化程度进行定量评价，有望成为一项定量评价阴茎海绵体纤维化病变的无创新方法。
Abstract
Objective To explore the feasibility of quantitatively evaluating the degree of penile cavernosa fibrosis in New Zealand white rabbits by shear wave elastography (SWE).Methods Twenty different ages New Zealand white rabbits with normal penile cavernosa and with penile cavernosa fibrosis were randomly selected for the penile SWE examination, with shear wave elastic quantitative measurement (SWQ) as the measurement index. After the SWE examination, the penile tissue was cut off for the HE staining, sirius red staining, and immunohistochemical staining. The following indicators reflecting the degree of penile cavernosa fibrosis were measured:the ratio of collagen fiber to smooth muscle cell (CF/SMC), the contents of fibronectin (FN), and collagen type Ⅲ (Col Ⅲ) in penile cavernous. The comparisons of age, bodyweight, and SWQ were performed between the two groups. Spearman correlation analysis was performed to analyze the correlation between the SWQ and CF/SMC, FN, and Col Ⅲ.Results There was no significant difference between the two groups in age and bodyweight, while there was a significant difference in SWQ (P < 0.05). SWQ was highly correlated with CF/SMC, FN, and Col Ⅲ (P all < 0.001), with the coefficients of the association being 0.760, 0.888, and 0.801, respectively.Conclusion SWE can quantitatively evaluate the degree of penile cavernosa fibrosis in New Zealand white rabbits, which means that SWE is expected to be a novel non-invasive method to quantitatively evaluate penile cavernosa fibrosis.
关键词
剪切波弹性成像(SWE) /
阴茎海绵体 /
纤维化 /
定量评价 /
兔
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Key words
shear wave elastography (SWE) /
penile cavernous /
fibrosis /
quantitative evaluation /
rabbit
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中图分类号：
R445.1
R697+.17
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脚注
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基金
复旦大学附属浦东医院引进人才科研启动金项目（YJRCJJ201804）；复旦大学医工结合科研项目（YG2021-035）；上海市血管病变调控与重塑重点实验室开放课题（zdsys202008）
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