A novel experimental model of New Zealand rabbit for the biocompatible assessment of 0.1 mm PTFE
ZHANG Hui-feng, YE Ming, YAN Xian-gang, CHEN Gang, TAO Qi-lin, JIA Bing△
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Department of Cardiovascular Surgery, Children's Hospital, Fudan University, Shanghai 201102, China
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文章历史+
收稿日期
出版日期
2016-06-21
2017-07-25
发布日期
2017-08-12
摘要
目的 建立评估0.1 mm聚四氟乙烯(polytetrafluoroethylene, PTFE)新型肺动脉瓣材料生物相容性实验的新西兰兔实验模型。方法 选取42只3 kg左右的新西兰成年兔,应用氯胺酮35 mg/kg和右旋美托咪啶0.25 mg/kg肌注麻醉,部分上段开胸,右心室流出道预置荷包,0.1 mm PTFE瓣膜材料经荷包中央小切口植入右心室,收紧荷包并打结完成操作,术后给予吸氧监护直至完全清醒。结果 42只新西兰成年兔接受了手术,死亡6只,其中2只死于麻醉药物的探索阶段,1只死于术后气胸,其余3只分别于术后5天、7天和8天死亡。经解剖发现植入瓣膜材料周围有血栓形成,调整术后抗凝治疗方案后无死亡发生。其余36只新西兰成年兔体重增长,完成整个实验过程。结论 新西兰兔适合作为0.1 mm PTFE瓣膜材料生物相容性研究的动物模型,优点是避免了气管插管和体外循环,气胸发生率低。
Abstract
Objective To establish a novel experimental model of New Zealand rabbit to assess the biocompatibility of 0.1 mm polytetrafluoroethylene (PTFE), a novel material of pulmonary valve. Methods Forty-two adult New Zealand rabbits about 3 kg were selected to give 35 mg/kg ketamine and 0.25 mg/kg dexmedetomidine intramuscularly for the anesthesia. The chest was open to expose the upper segment. The surface of right ventricular outflow tract (RVOT) was exposed after the pericardium was opened partially. The valve material (0.1 mm PTFE) was inserted into the right ventricle via the central mini-incision. Then the skin was closed and the rabbits were recoved with the oxygen inhalation. Results Forty-two adult New Zealand rabbits accepted the operation. Six rabbits died during the early period due to the inappropriate anesthesia drug (n=2), pneumothorax (n=1) and thrombosis (n=3), which occurred the 5th, 7th and 8th postoperative day. The anticoagulation treatment was adjusted to solve the thrombosis problem. Thirty-six rabbits survived for several months with weight increase until the experiment was finished. Conclusions The experimental model of New Zealand rabbit is appropriate for the biocompatible assessment of 0.1 mm PTFE. The advantage is to avoid intubation and cardiopulmonary bypass (CPB) and to decrease the pneumothorax.
ZHANG Hui-feng, YE Ming, YAN Xian-gang, CHEN Gang, TAO Qi-lin, JIA Bing△.
A novel experimental model of New Zealand rabbit for the biocompatible assessment of 0.1 mm PTFE[J]. Fudan University Journal of Medical Sciences, 2017, 44(04): 503 https://doi.org/10.3969/j.issn.1672-8467.2017.04.019