高效液相色谱法测定隐球菌性脑膜炎患者脑脊液中两性霉素B和伊曲康唑含量

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复旦学报(医学版) ›› 2008, Vol. 35 ›› Issue (6) : 866-0.

实验研究报道

高效液相色谱法测定隐球菌性脑膜炎患者脑脊液中两性霉素B和伊曲康唑含量

江峥1 朱利平2 于存静1 郁韵秋1Δ

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A Sensitive HPLC Method for Determination of Amphotericin B and Itraconazole in Cerebrospinal Fluid and its Application to Patients with Cryptococcal Meningitis

JIANG Zheng1, ZHU Li-ping2, YU Cun-jing1, YU Yun-qiu1Δ

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摘要

目的建立人脑脊液(CSF)中两性霉素B(AmB)和伊曲康唑(ITZ)含量测定的高效液相色谱方法(HPLC)，并用于隐球菌性脑膜炎患者脑脊液样品的测定。方法脑脊液样品经固相萃取(SPE)后直接进样。AmB测定采用Gemini 5w C18柱，流动相为5mmol/L乙二胺四乙酸二钠(醋酸调pH至4.5)/乙腈(62/38，V/V)，流速0.3mL/min，DAD检测器：检测波长410nm。ITZ测定采用YMC C18柱，流动相为0.2%三氟乙酸/乙腈(45/55,V/V)，流速1.1mL/min，荧光检测器：激发波长260nm，发射波长365nm。结果 AmB和ITZ的线性范围均为5ng/mL~100ng/mL，线性相关系数大于0.999，最低定量限为5ng/mL，低、中、高浓度质控样品在日内、日间精密度均小于7.6%。结论该方法灵敏、快速、简便，适用于隐球菌性脑膜炎患者治疗药物监测。

Abstract

Objective To establish a sensitive, rapid and simple high-performance liquid chromatography (HPLC) method for the determination of amphotericin B (AmB) and itraconazole (ITZ) in human cerebrospinal fluid (CSF). Methods Solid-phase extraction (SPE) was involved in sample preparation in this method, followed by reverse-phase separation under two different chromatographic systems. Results The linearity for both AmB and ITZ ranged from 5ng/mL to 100ng/mL and the inter-day and intra-day RSD were both below 7.6% at the concentrations of 20, 50 and 100ng/mL. Conclusions The method we established has been applied to the therapeutic drug monitoring on cryptococcal-meningitis-infected patients who were given comedication of these two drugs.

导出引用

江峥朱利平于存静郁韵秋Δ. 高效液相色谱法测定隐球菌性脑膜炎患者脑脊液中两性霉素B和伊曲康唑含量[J]. 复旦学报(医学版), 2008, 35(6): 866-0

JIANG Zheng;ZHU Li-ping;YU Cun-jing;YU Yun-qiuΔ. A Sensitive HPLC Method for Determination of Amphotericin B and Itraconazole in Cerebrospinal Fluid and its Application to Patients with Cryptococcal Meningitis[J]. Fudan University Journal of Medical Sciences, 2008, 35(6): 866-0

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收稿日期	修回日期	出版日期
1900-01-01	1900-01-01	2008-11-20
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2008-11-20

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