目的 建立同时分析血液中11种传统毒品及代谢物的液相色谱-串联质谱(LC-MS/MS)快速测定方法。方法 血液经乙腈沉淀蛋白后用Phenomenex Kinetex Biphenyl 100 Å(100 mm×3.0 mm×2.6 µm)色谱柱分离,以11种毒品对应的氘代标品及甲氧那明作为内标,采用多反应监测模式同时测定血液中11种传统毒品及代谢物。结果 各化合物检出限为0.5~2.0 ng/mL,定量限为1~5 ng/mL,在相应浓度范围内,11种传统毒品及代谢物均具有良好的线性关系,相关系数大于0.995,方法准确度为92.25%~109.56%,提取回收率为54.21%~101.86%,基质效应为79.60%~111.12%。结论 该方法选择性强、灵敏度高、所需样品量少(100 μL)、前处理过程简单快速(小于5 min),可用于毒驾等司法鉴定案件中11种传统毒品及代谢物的检验。
Abstract
Objective To establish a rapid method for the simultaneous analysis of 11 traditional drugs and metabolites in blood by liquid chromatography mass spectrometry (LC-MS/MS). Methods The blood was precipitated with acetonitrile and then separated with Phenomenex Kinetex Biphenyl 100 Å (100 mm×3.0 mm×2.6 µm) chromatographic column, with internal standard of methoxyphenamine and deuterated standard corresponding to 11 drugs. Eleven traditional drugs and metabolites in blood was detected by multi reaction monitoring mode simultaneously. Results The limit of detection of each compound was 0.5-2.0 ng/mL and the limit of quantitative was 1-5 ng/mL. Within the corresponding concentration range, 11 traditional drugs and metabolites had good linear relationship, the correlation coefficient was greater than 0.995, the accuracy of the method was in the range of 92.25%-109.56%, the recoveries were in the range of 54.21%-101.86%, and the matrix effect was 79.60%-111.12%. Conclusion The method has strong selectivity, high sensitivity and characterized by a low required sample volume (100 μL), a simple and fast pretreatment process(less than 5 min). It can be used to detect 11 traditional drugs and metabolites in forensic cases such as drug driving.
中图分类号:
R89
{{custom_clc.code}}
({{custom_clc.text}})
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] HICKS DL,RESKO SM,ELLIS JD,et al.Driving after cannabis use among young adults in Michigan[J].Cannabis Cannabinoid Res,2022,7(1):100-106.
[2] VAILLANCOURT L,VIEL E,DOMBROWSKI C,et al.Drugs and driving prior to cannabis legalization:A 5-year review from DECP (DRE) cases in the province of Quebec,Canada[J].Accid Anal Prev,2021,149:105832.
[3] XIANG X,WANG X,JIANG H,et al.Drugs and driving in China:Status and challenge[J].Int J Drug Policy,2016,31:203-204.
[4] JI KWON N,HAN E.A review of drug abuse in recently reported cases of driving under the influence of drugs (DUID) in Asia,USA,and Europe[J].Forensic Sci Int,2019,302:109854.
[5] SILBER BY,PAPAFOTIOU K,CROFT RJ,et al.The effects of dexamphetamine on simulated driving performance[J].Psychopharmacology (Berl),2005,179(3):536-543.
[6] GUSTAVSEN I,MØRLAND J,BRAMNESS JG.Impairment related to blood amphetamine and/or methamphetamine concentrations in suspected drugged drivers[J].Accid Anal Prev,2006,38(3):490-495.
[7] KOLODNY A,COURTWRIGHT DT,HWANG CS,et al.The prescription opioid and heroin crisis:a public health approach to an epidemic of addiction[J].Annu Rev Public Health,2015,36:559-574.
[8] VINDENES V,JORDBRU D,KNAPSKOG AB,et al.Impairment based legislative limits for driving under the influence of non-alcohol drugs in Norway[J].Forensic Sci Int,2012,219(1-3):1-11.
[9] SALAS-WRIGHT CP,CANO M,HODGES J,et al.Driving while under the influence of hallucinogens:Prevalence,correlates,and risk profiles[J].Drug Alcohol Depend,2021,228:109055.
[10] KELLY E,DARKE S,ROSS J.A review of drug use and driving:epidemiology,impairment,risk factors and risk perceptions[J].Drug Alcohol Rev,2004,23(3):319-344.
[11] 上海市公安局交通警察总队与司法部司法鉴定科学技术研究所.车辆驾驶人员体内毒品含量阈值与检验[Z].中华人民共和国公安部.2017:1-8.
[12] CHAN-HOSOKAWA A,NGUYEN L,LATTANZIO N,et al.Emergence of Delta-8 tetrahydrocannabinol in DUID investigation casework:method development,validation and application[J].J Anal Toxicol,2022,46(1):1-9.
[13] 彭毅侯,戚文杰,唐侃,等.基于公安部毒驾行业标准GA1333-2017的11中毒品LC-MS/MS检测方法[J].环境化学,2018,37(9):2084-2086.
[14] 琚妍妍,杨崇俊,刘冰洁,等.毒驾案件中17种毒品的超高效液相色谱串联质谱检测方法[J].刑事技术,2020,45(5):495-498.
[15] SCIENTIFIC WORKING GROUP FOR FORENSIC TOXICOLOGY.Scientific Working Group for Forensic Toxicology (SWGTOX) standard practices for method validation in forensic toxicology[J].J Anal Toxicol,2013,37(7):452-474.
[16] PETERS FT,DRUMMER OH,MUSSHOFF F.Validation of new methods[J].Forensic Sci Int,2007,165(2-3):216-224.
[17] AAFS Standards Board 2019,Standard practices for method validation in forensic toxicology,American Academy of Forensic Sciences[EB/OL].(2019-11-03)[2022-04-02]http://www.asbstandardsboard.org/wpcontent/uploads/2019/11/036_Std_el.pdf.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
基金
司法部司法鉴定重点实验室资助项目;上海市法医学重点实验室项目(21DZ2270800);上海市司法鉴定专业技术服务平台资助项目(19DZ2292700);上海市人才发展项目(2020102);国家自然科学基金(82130056)
{{custom_fund}}