CYP1B1基因多态性对前列腺癌根治术后生化复发(BCR)预测价值的初步研究

顾成元, 秦晓健, 戴波, 朱耀, 朱煜, 许华, 叶定伟

复旦学报(医学版) ›› 2020, Vol. 47 ›› Issue (01) : 19-23.

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复旦学报(医学版) ›› 2020, Vol. 47 ›› Issue (01) : 19-23. DOI: 10.3969/j.issn.1672-8467.2020.01.004
泌尿外科前沿论著专栏

CYP1B1基因多态性对前列腺癌根治术后生化复发(BCR)预测价值的初步研究

  • 顾成元, 秦晓健, 戴波, 朱耀, 朱煜, 许华, 叶定伟
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Genetic variants of the CYP1B1 gene as predictors of biochemical recurrence (BCR) after radical prostatectomy in localized prostate cancer patients

  • GU Cheng-yuan, QIN Xiao-jian, DAI Bo, ZHU Yao, ZHU Yu, XU Hua, YE Ding-wei
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摘要

目的 探讨CYP1B1基因多态性对前列腺癌根治术后生化复发(biochemical recurrence,BCR)的预测价值。方法 在426例行前列腺癌根治术的患者中采用Taqman探针法检测CYP1B1基因8个标签单核苷酸多态性(single nucleotide polymorphism,SNP)的分布情况。采用RT-PCR方法检测127例前列腺癌旁组织中CYP1B1 mRNA的表达。采用Cox比例风险模型和Kaplan-Meier法进行关联性和生存分析。结果 CYP1B1 rs1056836是前列腺癌根治术后BCR的独立预测因素(HR:0.65,95% CI:0.33~0.93,P=0.025),并且与CYP1B1 mRNA的表达相关。结论 CYP1B1 rs1056836有助于预测前列腺癌根治术后BCR的风险,其机制可能与影响CYP1B1基因表达相关。

Abstract

Objective To verify the hypothesize that genetic variants of CYP1B1 gene may influence biochemical recurrence (BCR) in clinically localized prostate cancer patients who received radical prostatectomy. Methods We genotyped eight tagging single nucleotide polymorphisms (SNPs) of CYP1B1 gene in 426 patients treated with radical prostatectomy (RP).The expression level of CYP1B1 mRNA in the adjacent normal prostate tissues was quantified by reverse transcription and real-time polymerase chain reaction (RT-PCR) in 127 cases.Kaplan-Meier analysis and Cox proportional hazard models were utilized to identify SNPs and its correlation with BCR. Results CYP1B1 rs1056836 was significantly associated with BCR (HR:0.65;95%CI:0.33-0.93,P=0.025) and CYP1B1 mRNA expression. Conclusions CYP1B1 rs1056836 may contribute to predicting the BCR of patients with RP,which may related to the genetic variation in CYP1B1 gene.

关键词

前列腺癌 / 生化复发(BCR) / CYP1B1 / 前列腺癌根治术 / 单核苷酸多态性

Key words

prostate cancer / biochemical recurrence (BCR) / CYP1B1 / radical prostatectomy / single nucleotide polymorphism

引用本文

导出引用
顾成元, 秦晓健, 戴波, 朱耀, 朱煜, 许华, 叶定伟. CYP1B1基因多态性对前列腺癌根治术后生化复发(BCR)预测价值的初步研究[J]. 复旦学报(医学版), 2020, 47(01): 19-23 https://doi.org/10.3969/j.issn.1672-8467.2020.01.004
GU Cheng-yuan, QIN Xiao-jian, DAI Bo, ZHU Yao, ZHU Yu, XU Hua, YE Ding-wei. Genetic variants of the CYP1B1 gene as predictors of biochemical recurrence (BCR) after radical prostatectomy in localized prostate cancer patients[J]. Fudan University Journal of Medical Sciences, 2020, 47(01): 19-23 https://doi.org/10.3969/j.issn.1672-8467.2020.01.004
中图分类号: R737.25   

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基金

国家自然科学基金(81702537)
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